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Samples were also taken on Days 2–6 at pre-dosing, on Day 7 at pre-dosing and 0.5, 1, 2, 2.5, 3, 4, 6, 8, 12, 24, 36, 48, and 72 h post-dosing. In Part 3 of the study drug-drug interaction with ritonavir was measured. In Period 1, blood samples were collected for BZF961 concentration on Day 1 at pre-dosing and 0.5, 1, 2, 2.5, 3, 4, 6, 8, 12, 24, and 36 h post-dose. In Period 2, blood samples were collected for BZF961 concentration on Day 2 at pre-dosing and 0.5, 1, 2, 2.5, 3, 4, 6, 8, 12, 24, and 36 h post-dose. Additionally, blood samples were collected for ritonavir concentration on Day 1 at 2 h post-ritonavir evening dose and on Day 2 at 1, 2, 4, and 8 h post-ritonavir morning dose. The plasma samples from all the subjects were assayed for BZF961 concentrations using a validated liquid chromatography-tandem mass spectrometry assay (LC-MS/MS). 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Mothers with surgical interventions are not evaluated here because these pregnant women were evaluated previously (10). As we previously mentioned, labour induction was also excluded from this analysis. There was no significant difference in the monthly distribution of infusions between controls and cases (χ28=7.55; p=0.48) and in the frequency of infusion during the second-third months of pregnancy (χ21=0.4; p=0.51).. Due to non-interventional nature of the study cytotec with out a prescription the treatment and follow-up procedures were left to the discretion of the physician, considering the fact that bisphosphonates are the first line choice in the treatment of postmenopausal osteoporosis in Turkey. The investigators were only asked to collect the study data in accordance with the principles of Good Epidemiological Practice.. Although several techniques have been reported to measure the thickness of masticatory mucosa we have chosen to sound the bone not only because it has been reported to be relatively reliable for measuring the tissue thickness [10], but also the same sites were used for harvesting grafts for gingival augmentation. The measurement error was reported to be 0.2mm with this direct technique [10], while those with other ″non-invasive″ methods were 0.54mm [6]. The possible disadvantages of the invasive methods have been an inspiration for the non-invasive methods such as ultrasonic devices [6, 15, 31], computed tomography [16], or CBCT [17, 18]. Although these methods may be less invasive, they have been reported to have difficulties such as obtaining consistently reliable results [6, 15, 31], overestimation of thickness values resulting from low contrast resolution of images [18] the high radiation exposure [16] or intra-oral manipulation [7]..
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