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A pilot study was conducted in advance of the present study. The pilot study involved an evaluation of lower‐jaw mobility of 10 patients during DGIM rounds in order to standardize lower‐jaw mobility measurements..
stage of memory making, which. Inclusion criteria were individuals aged 18–70 years; reading and writing literacy; having the ability to participate in various educational, cultural, financial, managerial activities; and at least 1 year of residence in Tehran. In the next stage, a sample of 15 experts was composed of managers and experts from the Center for Public Participation in controlling the epidemic of CAD. Selection criteria for experts included at least 5 years of management experience in centers related to the Center for Public Participation in controlling the epidemic of CAD in Tehran Province and having a willingness to cooperate. The data collection tool in this research is a researcher-made questionnaire of 35 questions and interviews made by reviewing the literature and documentation available at the Ministry of Health and Medical Education and the Department of Cardiology of the Center for Disease Control. Inclusion criteria were individuals aged 18–70 years; reading and writing literacy; having the ability to participate in various educational, cultural, financial, managerial activities; and at least 1 year of residence in Tehran. In the next stage, a sample of 15 experts was composed of managers and experts from the Center for Public Participation in controlling the epidemic of CAD. Selection criteria for experts included at least 5 years of management experience in centers related to the Center for Public Participation in controlling the epidemic of CAD in Tehran Province and having a willingness to cooperate. The data collection tool in this research is a researcher-made questionnaire of 35 questions and interviews made by reviewing the literature and documentation available at the Ministry of Health and Medical Education and the Department of Cardiology of the Center for Disease Control.. reframing process. They assist the subject in focusing on the selected. differentiation. P. cyanescens obviously represents a genetic. person refrain from supporting addictive .
All data are expressed as the mean ± standard error of the mean (S.E.M.). All experiments were repeated at least 3 times (≥ 3 replicates) on each specimen and there were 3 specimens from each group. The results of all replicates from each specimen were averaged, and the mean of averaged values from all specimens of a single group was regarded as the corresponding value of the whole group. Statistical analyses were performed using one-way ANOVA followed by Dunnett's post hoc analysis. The results were considered statistically significant if the p value was less than 0.05..
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Financial abuse is exploitation of or inattention to a person’s possessions or funds. It includes swindling, pressuring a person to distribute assets, and managing a person’s money irresponsibly.. Nucleic acid extraction, PCR and sequencing analysis: Nucleic acids (DNA/RNA) were extracted from frozen liver tissues and serum using SepaGene RV-R Kit (Sanko-Junyaku, Tokyo, Japan). The resulting pellet was resuspended in 100 µl RNase-free water, following the manufacturer's instruction. B19 DNA was screened by nested PCR using primers designed from VP1 region; PV1 (sense, 5'-GCT GTT AAG GAT GTT ACA GA-3', nucleotide (nt) 3520-3521) and PV1R (antisense, 5'-GGA TCC GTA TAA GGG ATT GT-3', nt 3882-3901) for the 1st PCR and PV2 (sense, 5'-CAG GTT ACT GAC AGC ACT AC-3', nt 3541-3560) and PV2R (antisense, 5'-TGT TGA CTG CAG CCC TCT AA-3', nt 3848-3867) for the 2nd PCR (Yoto et al., 1996). The sensitivity of this PCR assay allowed detection of as few as 10 copies of B19 genome. In addition, we sequenced the B19 isolates consisted of 4561 bp covering NS1, VP1 and VP2 regions obtained from liver tissues of patients. For this purpose, three overlapping PCR products (fragments A to C) were generated; for fragment A (1944 bp), PV3 (sense, 5'-TTT CCC GCC TTA TGC AAA TGG GCA G-3', nt 393-417) and PV5R (antisense, 5'-AGC TCC CAC ATG GCA GCT AC-3', nt 2533-2552) for the 1st PCR and PV4 (sense, 5'-TGT AAC GGT TAA AAT GGG CGG AGC G-3', nt 457-481) and PV6R (antisense, 5'- CCC CTT ACA CCG TCC CAC AC-3', nt 2382-2401) for the 2nd PCR; for fragment B (1835 bp), PV5 (sense, 5'-GCA GCA GTG GTG GTG AAA GC-3', nt 2143-2162) and PV1R for the 1st PCR and PV6 (sense, 5'-GGC GCC TGG AAC ACT GAA AC-3', nt 2208-2227) and PV2R for the 2nd PCR; for fragment C (1297 bp), PV1 and PV3R (sense, 5'-TAC AGT CTG GGT GGT ACT GGT GGG C-3', nt 5010-5034) for the 1st PCR and PV2 and PV4R (antisense, 5'-CTG GTG GGC GTT TAG TTA CGC ATC C-3', nt 4994-5018). Nucleotide positions are based on B19-HV isolate (accession No. AF162273). The PCR was done with AmpliTaq Gold (Perkin Elmer, Norwalk, Conn.) and Blend-Taq-Plus DNA polymerase (TOYOBO Co., Tokyo, Japan). The amplicons were separated by 1% agarose gel electrophoresis and purified using the QIAquick gel extraction kit (Qiagen Inc., Chatsworth, Calif.). Recovered amplicons were subjected to direct sequencing from both directions using the ABI PRISMTM Big Dye Terminator Cycle Sequencing Ready Reaction Kit (Perkin Elmer). Sequences of amplified DNA were determined using a sequencer (ABI model 373A; Applied Biosystems, Foster City, Calif.). Additionally, hepatitis A, B, C and E genomes were also determined by the nested PCR..