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For experiments where the acetate uptake was linear, the relative acetate uptake rate was quantified by dividing the uptake rate, i.e. the decay corrected signal increase per min, by the average signal derived from the reference area during incubation which provided a relative measure of the amount of [11C]-acetate added to the dish.. In our study, BrdU activity in the SLA group treated once with the LED was higher after 72 h than in the SLActive group, although the opposite result was observed with three LED applications. When cell count and BrdU results are taken into consideration, LED application at 0, 24, and 48 h appeared to slow down the osteoblast-like proliferation process and DNA activity. However, when LED was applied only once, cell proliferation and DNA activity in the early stages were significantly higher and a higher cell count was reached in all groups after 72 h, but DNA activity and live cell counts were found to be lower than in the SLA group. In our study, the high cell count after 72 h despite a decrease in DNA activity suggested that rapid cell proliferation occurred in the early phase. Aybar et al. [19] reached the same conclusion.. there?.

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