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Plasma was obtained from the rats and used to determine IL-1β and TNF-α concentration (pg/ml) using ELISA kits (Pierce Biotechnology, Inc.) (Catalogue number. IL-1β: ER2L1B. TNFα: ER3TNFA). The blood was obtained from the lateral vein of the tail (1 ml) prior anaesthesia. Plasma was obtained and for each test 100 µl was used..
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